4CMCFour Chaplains Memorial Chapel (Ft. Lewis, WA)
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1992), which was verified by a challenge with 4-chloro-m-cresol (4CmC, 100 [micro]M; Sigma-Aldrich).
To verify that ryanodine pretreatment in fact blocked all RyR channel activity in hippocampal neurons, a challenge of 4CmC [previously shown to activate RyR1 and RyR2, but not RyR3, channel activity (Fessenden et al.
Commonly used ryanodine receptor activator, 4-chloro-m-cresol (4CmC), is also an inhibitor of SERCA Ca2+ pumps.