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4E-BP14E-Binding Protein 1
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Abbreviations: CML, chronic myeloid leukemia; CQ, chloroquine; 3-MA, 3-methyladenine; ROS, reactive oxygen species; mTOR, mammalian target of rapamycin; NAC, N-acetyl-L-cysteine; MDR, multidrug-resistant; TKI, tyrosine kinase inhibitor; BCA, bicinchoninic acid; PVDF, poiyvinylidene fluoride; TEM, transmission electron microscopy; p70S6K, protein S6 kinase; 4E-BP1, 4E-binding protein 1.
Immunohistochemical stains were performed as previously described,24 using the following antibodies: CD79a (clone JCB117) and Bcl-2 (124; both from Dako, Carpinteria, California), PAX5 (24) and Bcl-6 (GL19E/A8; both from Ventana, Tucson, Arizona), CD20 (L26; Signet Laboratories, Dedham, Massachusetts), IRF-4 (MUM1; Santa Cruz Biotechnology, Santa Cruz, California), HHV8 LNA-1 (13B10; Novocastra, Newcastle upon Tyne, United Kingdom), Notch1 (mN1A; Chemicon International, Inc, Temecula, California), phospho-rpS6 (2215) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) (53H11; both from Cell Signaling, Danvers, Massachusetts), CD56 (CM164C; Biocare Medical, Concord, California), and cyclin D1 (RM-9104; Neomarkers, Fremont, California).
4EBP1: eukaryotic translation initiation factor 4E-binding protein 1; ATG1: autophagy-related protein 1; S6K: ribosomal protein S6 kinase; TSC1/2: tuberous sclerosis complex 1 and 2; ULK1: unc-51-like autophagy-activating kinase 1.