Prior to his initial surgery he had never had elevated 5HIAA
urinary levels and therefore this was not a useful marker to assess possible recurrence.
As these mutations affect synthesis of both serotonin and the catecholamines, concentrations of 5HIAA, HVA, and MHPG are, in general, significantly decreased in CSF (14).
Tyrosine hydroxylase deficiency leads to specific decreases in HVA and MHPG but normal levels of 5HIAA, as serotonin synthesis is unaffected (19).
Homicide, suicide and CSF 5HIAA
. Acta Psychiatr Scand.
2001), assessing the relative amounts of 5HT and its metabolite 5-hydroxyindole-acetic acid (5HIAA), with turnover calculated as the 5HIAA:5HT ratio.
The supernatant solution was collected, and aliquots were used for analysis of 5HT, 5HIAA, DA, HVA, and DOPAC by HPLC with electrochemical detection (Slotkin et al.
The results of the multiple regression revealed that the predictor variables entered the equation in the following order: (1) right frontal EEC accounted for 31% of the variance (p [less than] .001); (2) el evated serotonin (5HIAA
) accounted for an additional 13% of the variance (p [less than] .01); and (3) elevated cortisol, less positive interaction behavior, elevated norepinephrine, and low vagal tone added 7% to the variance (p [less than] .05).
The commonly used markers [alpha]-fetoprotein ([alpha]FP), carcinoembryonic antigen (CEA), CA19-9, and 5-hydroxyindoleacetic acid (5HIAA
) are all covered.
We here present a method that permits injection of an unprocessed urine sample and the simultaneous measurement of E, NE, dopamine (DA), metanephrine (M), normetanephrine (NM), 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA), as well as serotonin (5HT) and 5-hydroxyindole-3-acetic acid (5HIAA).
DA was obtained from Serva; DOPAC and HVA were purchased from ICN; and 5HIAA, M, NE, NM, and 5HT were from Sigma-Aldrich.
We routinely measure 5-hydroxyindoleacetic acid (5HIAA) by a long-established method using nitrosonaphthol, as first described by Uden-friend in 1955 , but with various modifications to improve specificity .
In our laboratory, all 5HIAA-positive urines are assessed by ultraviolet scanning of the reaction product and comparison with a 5HIAA standard before confirmation by HPLC at a neighboring laboratory.