Also found in: Dictionary, Thesaurus, Medical.
Copyright 1988-2018 AcronymFinder.com, All rights reserved.
References in periodicals archive ?
Separation of 6-TG and 6-MMP derivative was achieved with an 8-min isocratic elution with 95% mobile phase A-5% mobile phase B at a flow rate of 0.3 mL/min.
We determined intra- and interday imprecision by analyzing low, medium, and high concentrations of 6-TG and 6-MMP added to erythrocyte hemolysates.
The kinetics of conversion of thiopurine nucleoside monophosphates (6-TGMP and Me6-TIMP), diphosphates (6-TGDP and Me6-TIDP), and triphosphates (6TGTP and Me6-TITP) to 6-TG and 6-MMP derivatives was determined with nucleotide calibrators added to erythrocyte hemolysate at final concentrations of 10 [micro]mol/L for 6-TGNs and 100 [micro]mol/L for 6-MMPNs.
The first step consisted of simultaneous denaturation of the erythrocyte proteins and hydrolysis (100 [degrees]C for 1 h) of the 6-TGN to 6-TG by sulfuric acid (final concentration, 0.5 mol/L) in the presence of 2 mmol/L DTT to protect thiol groups from oxidation.
This was followed by hydrolysis (100 [degrees]C for 45 min) of the 6-TGNs in the separated supernatant to release the 6-TG. There was no further extraction or any other pretreatment of the sample before chromatography.
For chromatographic separation of the free 6-TG after both sample preparation procedures described above, we used a modification (7) of the Lennard chromatographic method (21).
6-TG and Me6-MP derivative liberated from the nucleotide moiety were analyzed by a reversed-phase HPLC method.
Retention behavior of 6-TG and Me6-MP derivative was not significantly modified by the pH of the mobile phase in the range 3.0 to 7.0.
The chromatogram of the erythrocyte sample supplemented with 6-TG and Me6-MP derivative is presented in Fig.