The chemical method has been replaced by enzymatic method for producing 7-ACA because of (1) safety and environmental contamination: dangerous and toxic reagents (i.
The biocatalytic conversion for CPC into 7-ACA, the key intermediate to cephalosporin derivates was developed (5).
The alternative enzymatic approach is a one-step enzymatic method process in which CPC is converted directly into 7-ACA by a CPC acylase (CA; Scheme 2).
This enzymes can hydrolyze GL-7-ACA to 7-ACA in high yield but have less activity against CPC; type 3 GL-7-ADCA acylase.
The natural of cephalosporin C acylase catalyzed the CPC to 7-ACA directly in a very low efficiency (14,15,16), significant substrate inhibition (14), and product inhibition (14).
The biotransformation of cephalosporin C to 7-ACA in one step enzymatic with cephalosporin acylase is a simple process with low cost and potential to applied in industrial production.