solstitialis on Daudi, A549
, HeLa, and Beas-2B were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide) assay.
As a result of mRNA relative expression analysis of Notch signaling genes in non-small cell lung cancer cell lines (H520, H1299, A549
and Calu-3) and in human bronchial epithelial cells, we obtained NOTCH1 and NOTCH2 receptors to be activated in all chosen cells.
In the present study, we found for the first time that BHS-I holds the promise to inhibit BaP-induced A549
lung cancer metastasis.
We adopted transcriptome sequencing of A549
cells treated with different doses of LPS and LXA4 and discovered N-myc downstream-regulated gene-1 (NDRG1) as a critical mediator of LXA4 and PI3K signaling to restore ENaC subunits' expression against LPS.
The cytotoxic effect of stigmasterol and stigmasterol derivatives were assayed with 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) method in three human cancer cell lines (MCF-7, A549
and HepG2) with some modification 25% trypsin would be digested during the phase of cells logarithmic growth and then made into cell suspension, the cell density was determined as:
These NPs are intended for pulmonary drug delivery of proteins and peptides and were already tested on A549
and 16HBE14o- cells applying a dose of 41.25 [micro]g, which was well tolerated.
Human adenocarcinoma A549
(ATCC# CCL-185) cell line was purchased from American Type Culture Collection (ATCC, USA).
Cell toxicity was evaluated on human alveolar cancer A549
cells, obtained from American Type Culture Collection (ATCC[R] CCL-185[TM]) and human embryonic stem Lep3 cells, obtained from the Laboratory of Cell Cultures, National Centre of Infectious and Parasitic Diseases, Sofia, Bulgaria.
McCoy, mice fibroblast cell line, and A549
a human adenocarcinoma-derived type II alveolar epithelial cell line were maintained in continuous culture in EMEM growth media and Ham's F-12K (Kaighn's) growth media, respectively, with 10% of FBS and supplemented with penicillin (100 units x [ml.sup.-1]) and streptomycin (100 mg x [ml.sup.-1]).
Yahaya, "Antiproliferative activity of clinacanthus nutuns water extract through human lung cancer cell (A549
)," in Proceedings of the 2nd AMDI International Biohealth and Sciences Conference, Kompleks Cahaya Siswa USM Penang, January 2016.
Two cybrids within the adenocarcinoma A549
nuclear background were intended to harbor the m.8993T (Awt) or m.8993G (Am) mtDNA alleles.
Our previous study demonstrated that PSO inhibited the proliferation of A549
cells mediated by autophagy through ROS generation (Hao et al.