Human adenocarcinoma A549
(ATCC# CCL-185) cell line was purchased from American Type Culture Collection (ATCC, USA).
Furthermore, we explored the in vitro effects of small-interfering RNA (siRNA)-mediated NEDD4-1 knockdown on cell proliferation, migration and invasion, drug sensitivity, and PI3K/Akt pathway in the human A549
Lee, Anti-Metastatic Effect of Cantharidin in A549
Human Lung Cancer Cells, Arch.
Nine HAdV positive environmental swabs were inoculated into A549
cells, and no cytopathic effect was observed after one blind passage, therefore the presence of live virus could not be confirmed.
C2 ceramide, Ceranib-2, Human lung adenocarcinoma cells, A549
human lung cancer cell lines were used in this study.
Two cybrids within the adenocarcinoma A549
nuclear background were intended to harbor the m.
Our previous study demonstrated that PSO inhibited the proliferation of A549
cells mediated by autophagy through ROS generation (Hao et al.
For most tumor cell lines 5,000 cells per well is required to perform proliferation assays, hence viable cells of cell lines A549
and NCIH520 were seeded in 96-well plates in RPMI-1640 Medium and were kept for incubation overnight.
Assay method for cytotoxic activity: The cytotoxicity against A549
, HCT116, Hep G2, and MCF7 cells was tested in the National Cancer Institute based on the SRB (Sulforhodamine B) assay using 3-(4,5-dimethylthiazol-2yl)-2,5- diphenyltetrazolium bromide (MTT) method given by Skehan et al.
Methods: The case-control study was conducted at the First Affiliated Hospital of Nanchang University from 1st January to 30th June, 2014 and comprised A549
cells which were treated with a series of concentrations of nicotine (0.
Cell Cultures: A549
cells were acquired from the American Type Culture Collection (ATCC, Rockfield, MD) and grown in Ham's F12K media supplemented with 10% fetal bovine serum (Sigma-Aldrich) and 1% antibiotic/antimycotic solution (Corning).