A549human alveolar epithelial cells
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Reagents: F-12K, Dulbecco's Modified Eagle's (DMEM) culture media, Phosphate- buffered saline (PBS), Trypsin EDTA, Fetal bovine serum (FBS), Penicillin-Streptomycin, A549 human lung cancer cell line, and MRC-5 human normal lung cells line were each purchased from American Type Culture Collection (ATCC) located in Manassas, VA, USA.
For analysis of TrxR activity in cultured cells, A549 cells were cultivated as monolayers in 75- [cm.
Results: Phosphorylation of ERK1/2 in A549 cells was increased by exposure to 95% [O.
Rapid PCR was initially tested using cDNA synthesized from A549 RNA mixed with RNA from a lymph node that was histologically positive for metastatic esophageal cancer.
A recent report described that the antitumor activity of capillin was much more potent in epidermoid carcinoma of the larynx HEp-2 cells than in other cancer cells, such as colon carcinoma HT29, pancreatic carcinoma MIA PaCa-2, and lung carcinoma A549 (Whelan and Ryan 2004).
Although A549 cells have been proposed as a standardized model to study lung epithelium (Foster et al 1998), they are tumorous cells that may respond differently than primary cell lines.
Additional control reactions were performed in parallel and contained all reaction components with the following changes; no-template control mixtures lacked template RNA, and negative control mixtures contained RNA from A549, MEG-01, or healthy PBLs only.
DelMar has presented data demonstrating that, in an established murine xenograft model of NSCLC, the activity of VAL-083 was compared to standard platinum-based therapy with cisplatin against human NSCLC cell lines A549 (TKI-sensitive) and H1975 (TKI-resistant).
2010) established the effects of formaldehyde exposure on DNA-damage protection on human A549 alveolar epithelial cells.
We determined the effects of theaflavin, a black tea-derived polyphenol, on tumor necrosis factor-alpha-mediated expression of the interleukin-8 gene in A549 cells.