According to the relative expression results which were analyzed by a Relative Expression Software Tool (REST) and AACt
method, there is an overexpression rate of 8 fold in CCR5 expression in comparison to mock-treated group (p<0.001).
Values are calculated as fold change with respect to healthy samples with the AACt
(5.) Gosselin S, Morris M, Miller-Nesbitt A, Hoffman RS, Hayes BD, Turgeon AF, Gilfix BM, Grunbaum AM, Bania TC, Thomas SH, Morais JA, Graudins A, Bailey B, Megarbane B, Calello DP, Levine M, Stellpflug SJ, Hoegberg LC, Chuang R, Stork C, Bhalla A, Rollins CJ, Lavergne V; AACT
Lipid Emulsion Therapy workgroup.
uidA gene expression was calculated by the AACt
method using non-inoculated transgenic plants as calibrators (LIVAK & SCHMITTGEN, 2001).
Then, the former was subtracted by the latter, namely AACT
. The value of 2 - AACT
, the fold change of gene expression, was plotted into the figure.
Relative differences between groups were calculated based on the equation relative quantification = 2 - AACt
The relative fold change in gene expression was evaluated using the comparative threshold cycle AACt
The differential level was calculated using the AACt
The final results were presented as the ratios of the relative amount of the target gene (TNF-[alpha]) to the control gene (GAPDH) using the 2-AACT equation, where AACT
= CT (target gene) - CT (internal control gene), according to the method described by Livak and Schmittgen .
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Samples were assayed in triplicate and the relative abundance of mRNAs was calculated with the AACT
method with [beta]-actin as the invariant control.