The MTT reduction assay was used to measure neuroprotection of AAFM against oxidative stress induced by hydrogen peroxide.
The LDH assay was used to verify nerve cell membrane protection of AAFM. The measurement of endoenzyme such as LDH amount released into the medium can be used to evaluate the degree of nerve cell membrane damage.
Mice were randomly assigned to five groups: control (water-oral administration + saline-injection) group, TMT (water-oral administration + TMT-injection) group, and AAFM (AAFM-oral administration + TMT-injection) groups (5, 10, and 20 mg/kg of body weight, resp.).
UPLC QTOF/MS was used to analyze the quality of main compounds in the AAFM. An electrospray source in negative ion mode was used to obtain MS and MS/MS data.
To measure the protective effect of AAFM against [H.sub.2][O.sub.2]-induced oxidative stress, DCF-DA assays were carried out to confirm ROS production.
These results showed that the antioxidant activity of the phenolic compounds from AAFM protected the cells against [H.sub.2][O.sub.2]-induced oxidative stress.
Improving Effect of AAFM on TMT-Induced Cognitive Dysfunction.
This contract reportedly provides a good platform for the further internationalisation of the activities of AAFM, a joint venture of ARCADIS and Carillion (LSE:CLLN), while at the same time it strengthens the firm's position in the Benelux market.
Under the terms of the contract, financial details of which were not disclosed, AAFM will provide an integral package of facility management services for all the office locations of BT in the Netherlands, Belgium and Luxemburg.
Colonel Charlie Simpson, USAF (Ret.), Executive Director of the Association of Air Force Missileers (AAFM
), "Victors in the Cold War."