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This finding demonstrates the successful start-up of the CANON process in the SBR and indicates that the inoculation of AAOB biomass can significantly shorten the start-up time of the CANON process.
Additionally, it was observed that nitrite concentrations were very low in the subsequent three nonaeration times, which limited the activity of AAOB and resulted in unchanged ammonia concentrations.
This finding suggests that the ammonia conversion rate and the nitrate production rate decreased with decreasing aeration times, which were also related to the activities of AOB, NOB, and AAOB. The CANON process relied on the incorporation of AOB and AAOB, while NOB was not expected in the reactor.