The metabolic conversion of resazurin to resorufin was used as a measure for viability, which was estimated 1 h and 24 h post-treatment with AAPH
and UV radiation, respectively.
DNA breaking activity of the carbon-centered radical generated from 2,2'azobis-(2-amidinopropane)-hydrochloride (AAPH
This kinetic assay is based on the measurement of radical scavenging activity of extract against peroxyl radicals produced by the addition of AAPH
Then, 50 mM 2,2'Aazobis(2-amidinopropane) dihydrochloride (AAPH
) solution was added.
3-(4,5-Dimethyl-2-thiazolyl)-2,5- diphenyltetrazolium bromide (MTT) and dimethyl sulphoxide (DMSO), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azobis(2-methylpropionamidine)-dihydrochloride (AAPH
), fluorescein disodium salt, and 2,4,6-Tris (2-pyridyl)-1,3,5-triazine (TPTZ) were provided by Sigma-Aldrich (St.
Bakken, "Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by [H.sub.2][O.sub.2] and AAPH
," Journal of Food Science and Technology, vol.
Para el desarrollo de la tecnica se utilizaron soluciones de fluoresceina 1x[10.sup.-2] M en PBS (Buffer de Fosfato de Sodio 75 mM) y AAPH
0,6 M en PBS (Buffer de Fosfato de Sodio 75 mM).
School of Public Health, Departments of Nutrition ; Epidemiology ; Global Health and Population, USA ; Africa Academy for Public Health (AAPH
), Tanzania .
Set the program of fluorescence / chemical analyzer, 37 min incubation 20 mmol/L, automatic mixing, quickly add 20 AAPH
L (119.4), automatic mixing and began to measure.
Subsequently, the RBC suspension was mixed with different concentrations of teas with 2, 2'-azobis (2-amidinopropane) hydrochloride (AAPH
(6.) Brand PLP, Vaessen-Verberne AAPH
. Differences in management ofbronchiolitis between hospitals in the Netherlands.
A solution of 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH
) was prepared before the test.