(10,11,12,13,14) In addition, certain clinical studies have been conducted on the use of PRF for treatment of intra-bony defects in humans, (15,16,17,18,19) but none have investigated its additive effects with ABBM for treatment of intra-bony defects.
The selected sites were randomly (coin toss) divided into control (ABBM alone) and test (ABBM-PRF) groups.
(28) One PRF was cut by scissors to produce small pieces and then mixed with ABBM granules with a particle size of 0.25-1 mm (Bio-Oss, Gesitlich, Switzerland) in a sterile dappen dish; this mixture was added to the defect by means of a sterile curette.
In the control group, ABBM was prepared by pouring the particles into a sterile dappen dish and rehydrated with sterile saline, and graft material was placed on the defect; the defect was filled to the existing alveolar crest.
The present randomised clinical trial compared the use of ABBM alone (control group) to ABBM-PRF combination (test group) in surgical treatment of intra-bony defects.
To the best of our knowledge, to date, there have been no published reports which compare clinical and radiographical effects of ABBM with those of ABBM-PRF in the treatment of periodontal intra-bony defects.
(30) compared ABBM with ABBM-PRP in human intra-bony defects and reported no significant differences between the two treatment groups.
In terms of vertical bone gain, the results of this study compare favourably with those reported in previous human studies with ABBM. (32, 35) Conversely, the reduction in the depth of the defect obtained in the present study is lower compared to the reductions observed in a previous study which reported a 3.8-mm defect fill at the 18-month evaluation of defects treated with a DFDBA-PRP combination.
In previous studies, the control groups were treated with conventional open-flap debridement alone or with PRF, whereas in the present study, the control group was treated with ABBM. ABBM is an extensively researched material which has been successfully used alone for treatment of intra-bony and furcation defects, with a positive role in reducing PD, promoting CAL gain and enhancing defect fill in periodontal defects.
Many other important activities were organized, such as the ABBM
Award for Students (in two categories: undergraduate and graduate students), the photography contest, parallel scientific meetings, film exhibitions, and the special award for outstanding researchers on Marine Biology (Medalha Biologia Marinha Brasil), among others.