Programa Nacional de Melhoramento do Guzera para Leite: resultados do Teste de Progenie, do Programa de Melhoramento Genetico de Zebuinos da ABCZ
e do Nucleo MOET.
a) Alem da disputa politica na ALMG, a medalha a 'Stedile' gerou notas de repudio assinadas pela Sociedade Rural Brasileira (SRB) e pela Associacao Brasileira dos Criadores de Zebu (ABCZ
), sediada em Uberlndia;
In addition to solar energy firm ABCZ
Solar LLC, the couple also owns land development company ABCZ
Properties LLC, charter jet firm Fly Private LLC and Hurricanes Investment LLC, an ownership partner in the Carolina Hurricanes NHL hockey team.
The measurement of scrotal circumference has been added to the database of ABCZ
over the last years and the small amount of data and/or the short period was the most likely reason for the absence of a significant trend.
(ASSOCIACAO BRASILEIRA DOS CRIADORES DE ZEBU).
Fragments from 7 housekeeping genes (abcZ
, adk, aroE, fumC, gdh, pdhC, andpgm) were used for typing, as given on the Neisseria MLST website (http://pubmlst.org/neisseria/).
Foram utilizados 12.450 pesos padronizados aos 205 (P205) dias de idade de bovinos da raca Nelore, nascidos entre 1976 e 2006, incluidos no controle de desenvolvimento ponderal da Associacao Brasileira de Criadores de Zebu (ABCZ
The third isolate had a novel allele at the abcZ
locus and was assigned ST-4242.
MLST was performed by determining the nucleotide sequences of 7 housekeeping genes (abcZ
, adk, aroE, fumC, gdh, pdhC, pgm) as previously described (8).
MLST was performed by using the following seven housekeeping genes (protein products are shown in parentheses): abcZ
(putative ATP-binding cassette transporter), adk (adenylate kinase), aroE (shikimate dehydrogenase), fumC (fumarate hydratase) gdh (glucose-6-phosphate dehydrogenase), pdhC (pyruvate dehydrogenase subunit), and pgm (phosphoglucomutase) as previously described (6).
PFGE was done by digesting chromosomal DNA prepared in agarose blocks with NheI and SpeI as described (22), and MLST by sequencing gene fragments of abcZ
, adk, aroE, fumC, gdh, pdhC, and pgm, also as described (16; http://www/ mist.net).
For MLST, the primers of the housekeeping genes abcZ
(putative ABC transporter), adk (adenylate kinase), aroE (shikimate dehydrogenase), gdh (glucose-6-phosphate dehydrogenase), pdhC (pyruvate dehydrogenase subunit), and pgm (phosphoglucomutase) were synthesized by our institute according to the sequences published by Maiden et al.