These findings exclude the possibility that the young Vietnamese girl was infected with ABLV
, now known to be endemic within Australian bats, after she arrived in Australia.
Initially, all 394 samples were screened in a modified rapid fluorescent focus inhibition test (6) against rabies virus (RABV, strain CVS-11) and ABLV (pteropid subtype; 40 50% tissue culture infective dose), with World Health Organization standard serum as a source for positive control antibody with 50% endpoint dilution of 1 IU = 1:20.
All 394 serum samples were negative against RABV, but 16 (4%) were positive or suggestive of ABLV (Table 2).
This study presents evidence of neutralization of lyssaviruses other than RABV and ABLV by sera from Thai bats.
Furthermore, neutralizing antibodies against ABLV were detected in the Philippines in two frugivorous species and four insectivorous species, notably M.
Antigens were obtained from inactivated and titrated supernatants of BHK21 clone BSR cell cultures infected independently by tbur different genotypes circulating in bats, RABV (CVS strain), LBV, ABLV, and EBLV-1.
detected neutralizing antibodies against ABLV in the serum of six bat species (Mineopterus schreibersi, Taphozous melanopogan, Philetor brachypteus, Scotophilus kuhli, Pteropus hypomelanus, and Rousettus amplexicaudatus) in the Philippines (29).
Then genotype 1 was represented by seven rabies viruses (SRL1032, 86118BRE, 1500AFS, 9218TCH, 8738THA, insectivorous bat/Chile, and PA R89), and genotypes 2, 3, 4, 5, 6, and 7 were represented by Lagos bat virus (8619NGA), Mokola virus (MOK/U22843), Duvenhage virus (86132AS), EBLV-1 (8918FRA), EBLV-2 (9007FIN), and ABLV (Balina/AF006497), respectively (Table 2).
As many bats involved in such incidents are uninfected, and a negative results obviates the need for postexposure prophylaxis, determining each bat's ABLV infection status is preferable.
A case is described in which circumstantial evidence exists for bat-to-bat cross-species transmission of ABLV.
ABLV and classic Rabies virus (RABV) are members of the genus Lyssavirus.
This monoclonal antibody preparation reliably detects infection with all known lyssaviruses, including both classic RABV and ABLV (5,15-17).