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4] Human genes: GALC, galactosylceramidase; ACADM, acyl-CoA dehydrogenase, C-4 to C-12 straight chain; ACADVL, acyl-CoA dehydrogenase, very long chain; GALT, galactose-1-phosphate uridyl transferase; PAH, phenylalanine hydroxylase; FCGR2A, Fc fragment of IgG, low affinity IIa, receptor (CD32); RPP30, ribonuclease P/MRP 30kDa subunit.
c) Population study of samples retained for interesting genotypes of ACADM, coagulation (F2, F5, MTHFRR and warfarin susceptibility (VKORC1, CYP2C19), courtesy of Associated Regional and University Pathologists and BioFire Diagnostics.
Primers were designed for PPAR[gamma], FASN, ACADM, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes using Primer Express 3.
Total RNA from different adipose tissues, LD and other organs were analyzed by qRT-PCR for the following genes: PPAR[gamma], FASN, ACADM.
PPAR[gamma], FASN, and ACADM mRNA expression in adipose tissue and LD of Yanbian yellow cattle
PPAR[gamma], FASN, and ACADM were expressed in adipose tissues and LD of Yanbian yellow cattle, as shown in Figure 2, 3, and 4.
FASN and ACADM mRNA expressions were detected in the same samples, though FASN gene showed a trend for higher expression in adipose tissue than in LD (Figure 3); ACADM gene showed a trend for higher expression in adipose tissue than in LD, though the difference was not statistically significant (Figure 4).
PPAR[gamma], FASN, and ACADM mRNA expression levels in adipose tissues and LD of Yan yellow cattle
As shown in Figure 2, 3, and 4, PPAR[gamma], FASN, and ACADM mRNA expression were detected in Yan yellow cattle.
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