ACBRIApplied Cell Biology Research Institute
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number ACBRI 181, Kirkland, WA, USA) were purchased from Cell Systems with CSC complete medium (glucose concentration of 5 mmol/L), and cells at passage 3-7 were used in the following experiment.
HRMVECs (catalog number ACBRI 181) were purchased from Cell Systems (Kirkland, WA) and grown in Medium 131 containing microvascular growth supplement (Gibco, Life Technologies Corp., Carlsbad, CA), 50 U/mL penicillin, and 50 [micro]g/mL streptomycin at 37[degrees]C, 95% air, and 5% C[O.sub.2].