A survey of databases containing sequences of hyperthermophilic genomes indicated that none of the hyperthermophilic genome sequences released so far, including the 17 fully sequenced genomes of Thermococcales, bear a gene with homology to any of the well-known acetaldehyde-producing enzymes PDC or AcDH (CoA-acetylating).
Each genome of hyperthermophilic archaea and bacteria was separately searched for annotations, and BLAST searches were performed for homologs of the each type of the CoA-dependent AcDH. In addition, CoA-dependent acetaldehyde dehydrogenase activity under the conditions examined (different pHs and buffers, different amounts of CFEs, and using NAD+ and NAD[P.sup.+] as cofactors) was not detectable in CFEs of two hyperthermophilic archaea, T.
There are two possible pathways for the production of acetaldehyde, which are (a) the consecutive reactions of POR and/or pyruvate formate lyase (PFL) followed by AcDH (CoA-dependent) and (b) the direct production of acetaldehyde from pyruvate by the enzyme PDC (Figure 6), though the former is unlikely as a CoA-dependent acetaldehyde dehydrogenase activity was not detected.
The AcDH (CoA-acetylating) encoding genes were absent from the released genome sequences of hyperthermophiles.
Dado que los compromisos en derechos humanos adquiridos por los Estados miembros de la OMC no se suspenden en el contexto de esta organizacion, es claro que, a traves de la OMC, los paises integrantes deben contribuir a la realizacion de sus responsabilidades en materia de derechos humanos (
ACDH 2003).
