Short PCR amplicons (<240 bp; see Table 1 in the online Data Supplement) targeting different regions of the ACTB
and DDX4 transcripts were detected in cfsRNA samples from all 9 volunteers; however, we detected long PCR amplicons of ACTB
mRNA (1499 bp) in 2 of the 9 volunteers but detected no long amplicons for DDX4 mRNA (1909 bp).
Subjects] was negligible for ACTB
and CASP3 and was approximately 1 cycle for IL1B and IFNG.
No test rate was calculated based on the Ct cutoff for ACTB
However, transcript abundance of reference genes, including ACTB
, GAPDH, HPRT, and PPIA genes significantly changed when RNA was isolated from the muscle stored on ice for 1.
8] Human genes: ERCC1, excision repair cross-complementation group 1; EPCAM, epithelial cell adhesion molecule (also known as GA733-2); MUC1, mucin 1, cell surface associated; MUC16, mucin 16, cell surface associated (also known as CA125); ACTB
, actin, beta; PPIC, peptidylprolyl isomerase C (cyclophilin C).
will house select UIC faculty from chemistry, biology and physics and support cutting-edge research focused on connections between chemistry, physics and biology, such as tumor growth, HIV/AIDS, immunology, dental services, orthopedics, laser dynamics, nanoscience and environmental science.
The amendment to section 355's ACTB
rule was generally effective as of the date of the enactment of the legislation.
We recorded PCR results from the 7500 Fast Dx software for ACTB
and methylated SEPT9 for each of the triplicate reactions.
mtDNA content is determined as mtDNA copy number (mean of MTF3212/R3319 and MT-ND1) normalized to nDNA copy number (mean of RPLPO, ACTB
777 in whole blood and PBMC, respectively), while ACTB
the least stable one with the highest M value (0.
All analyses were performed in triplicate, and the gene expression levels were normalized to the housekeeping gene ACTB
5) Human genes: TBP, TATA box binding protein; UBC, ubiquitin C; SDHA, succinate dehydrogenase complex, subunit A, flavoprotein (Fp); RLP13, receptor like protein 13; YHWAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; HMBS, hydroxymethylbilane synthase; B2M, fi-2 microglobin; hypoxanthine phosphoribosyltransferase 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RPS18, ribosomal protein S18; ACTB
, actin, beta; GSS, glutathione synthetase (hGus); 36B4, acidic ribosomal phosphoprotein P0.