MenSCs (passage 3) were seeded in 24-well plates (5 x [10.sup.4] cells/well) and infected with the adenoviral vectors AdGL, AdGL-RGD, and AdGLK at different multiplicity of infection (MOI 25,10, 5, and 1 TU/cell) in triplicates during 24 hours at 37[degrees]C.
MenSCs were infected with different amounts of AdGL (wild-type capsid), AdGLRGD (insertion of Arg-Gly-Asp (RGD) in the HI-loop of the fiber knob), and AdGLK (replacement of the KKTK fiber shaft heparan sulfate glycosaminoglycan-binding domain with an RGDK motif).
Os acidos araquidonico, di-homo-gama-linoleico (20:3 n-6, ADGL
), e eicosapentaenoico (20:5 n-3, EPA) sao precursores dos prostanoides das series 1, 2 e 3 e dos leucotrienos das series 4, 5 e 6, respectivamente.
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