Dietary Vitamin [D.sub.3] Did Not Affect the Expression of CCR4 or CCR10 on Cells in the SDLN or ADLN. Dietary vitamin [D.sub.3] could induce the migration of [T.sub.Reg] cells to augment the percentages of these cells in the SDLN, facilitated by the expression of chemokine receptors.
These observations were accompanied by a lack of effect of dietary vitamin D on the suppressive activity of cells from the ADLN. This curious observation is difficult to explain but may represent a site from which [T.sub.Reg] cells actively migrate (to the SDLN).
Furthermore, reduced percentages of [T.sub.Reg] cells were observed in the ADLN of mice fed a vitamin [D.sub.3]-replete diet.
1,25[(OH).sub.2][D.sub.3]: 1,25-Dihydroxyvitamin [D.sub.3] 25(OH)[D.sub.3]: 25-Hydroxyvitamin [D.sub.3] ADLN: Airway-draining lymph nodes DNFB: 2,4-Dinitrofluorobenzene [T.sub.Eff] cells: Effector T cells MLN: Mesenteric lymph nodes [T.sub.Reg] cells: Regulatory T cells SDLN: Skin-draining lymph nodes VDBP: Vitamin D-binding protein.
(a) The FACS gating strategy for determining the percentage of CD3+CD4+CD25+Foxp3+ ([T.sub.Reg] cells, orange) or CD3+CD4+CD25+Foxp3([T.sub.Eff] cells, yellow) cells in skin, SDLN, lung, ADLN, MLN, spleen, and blood.
In (a), the number of cells (x107)/mouse or mL of blood isolated from the SDLN, ADLN, MLN, spleen, or blood is shown.
CD4+CD25+(Foxp3+) cells were purified from (a) SDLN, (b) ADLN, (c) MLN, (d) spleens, or (e) blood of mice and cocultured with lymph node responder cells from D011.10 mice at ratios of 1: 8,1: 16, or 1: 32 and OVA peptide.
Caption: Figure 5: Dietary vitamin [D.sub.3] did not modify neuropilin expression on Foxp3+ [T.sub.Reg] cells from the skin, SDLN, lung, or ADLN or alter the capacity of DCs from the ADLN to induce new Foxp3+ [T.sub.Reg] cells.