: adipose mesenchymal stem cell; PC1: Prohormone Convertase 1; ILC-2: group 2 innate lymphoid cells; BMP7: bone morphogenic protein 7; EID1: EP300-interacting inhibitor of differentiation 1; M2 ATM: adipose tissue macrophage 2; FGF21: fibroblastic growth factor 21; PPARg: peroxisome proliferator-activated receptor gamma; TR: thyroid receptors; FXR: farnesoid X receptor; BNP: brain natriuretic factor.
were isolated as described previously .
While a number of collagenases are available for ADMSC
isolation (Table 1), type 1 collagenase is the preferred agent for isolation prior to chondrogenic lineage induction.
Recombinant human fibroblast growth factor basic/2 (FGFb) has been shown to have positive osteogenic effects on human BMSCs and human ADMSCs
by enhancing osteogenic differentiation in vitro and bone osteoid area in vivo .
Wallace et al., "Intravenous administration of xenogenic adipose-derived mesenchymal stem cells (ADMSC
) and ADMSC-derived exosomes markedly reduced brain infarct volume and preserved neurological function in rat after acute ischemic stroke," Oncotarget, vol.
: adipose mesenchymal stem cell; PC1: Prohormone Convertase 1; ILC-2: group 2 innate lymphoid cells; BMP7: bone morphogenic protein 7; M2 ATM: adipose tissue macrophage 2; FGF21: fibroblastic growth factor 21; PPARy: peroxisome proliferator-activated receptor gamma; TR: thyroid receptors; FXR: farnesoid X receptor; BNP: brain natriuretic factor.
The technology yields adult autologous AdMSCs
in quantities never possible for use in therapies for vascular, autoimmune, and degenerative diseases, as well as injuries.
The DPMSCs, UCMSCs, and ADMSCs
were checked for their surface marker profile by FACSCalibur flow cytometry system (Becton Dickinson, CA, USA) as already described [8, 9, 23].
Biomat)  (5 x [10.sup.6]/ implant) Yoon et al., 2015 ADMSCs
(J Vet Sci)  (1 x [10.sup.6]/ 50 [micro]l) Berner et al., BMSCs 2015 (Stem cells (100 x Trans Med)  [10.sup.6]) Masaoka et al., BMSCs 2016 (The Open (1.3-4.1 x Biomed Eng J) [10.sup.6]/ml)  Smith et al., BMSCs 2017 (J Tiss Eng (1 x [10.sup.7]/ Reg Med)  implant) Kirker-Head BMP-2 et al., 1995 (2 mg and 4 mg) (Clin.
Adipose derived mesenchymal stem cells (ADMSCs
) are an attractive source for regenerative medicine because they are abundant and easy to obtain from liposuction aspirates or excised fat .
As trial sponsor, Celltex will provide adult autologous adipose tissue -derived mesenchymal stem cells (AdMSCs
) cultured and expanded by Celltex's proprietary stem cell process
Adipose-derived mesenchymal stem cells (ADMSCs
) have been used for damaged cartilage regeneration due to adipose tissue that can provide an abundant source of ADMSCs
autologously and does not pose the ethical, tumorigenic, or immunogenic risk as presented by pluripotent stem cells.