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ADRB2Beta-2-Adrenergic Receptor (also seen as B2AR)
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The ADRB2 genomic DNA and amino acid sequences of various species (horse, human, chimpanzee, mouse, rat, cow, pig, chicken, dog, and cat) were retrieved from the Ensemble database (http://www.
It seems reasonable to incorporate 2 bulges for haplotyping of a 3-loci block, as shown with the ADRB2 20/ 46/79 probe, because only 4 haplotypes have been described at this locus.
For the genes NQO1, TNFA, TLR4, and ADRB2, we selected tag single-nucleotide polymorphisms (SNPs) in the gene region, 10 kb upstream from the 5' untranslated region (UTR), and 10 kb downstream from 3' UTR.
The SNP genotypes were determined by probe-extension reactions using two probes with different base sequences at their 3' termini: rs1042718 on the ADRB2 gene (5'-CCC ATT CAG ATG CAC TGG TTC N-3', where N = A or C); rs3918242 on the gelatinase B gene (5'-CTC CCG AGT AGC TGG TAT TAT AGA CN-3', where N = G or A); rs1346044 on the WRN (5'-CTC CTT TTG TTG ACA TCT CN-3', where N = A or G); and rs1801133 on the MTHFR gene (5'-CTT GAA GGA GAA GGT GTC TGC GGG TGN-3', where N = C or T).