The main aflatoxins naturally occurring in foods are designated as AFB1, AFB2
, AFG1, and AFG2.
In the EU, Current maximum levels for total aflatoxins in groundnuts, cereals and dried fruits (AFB1, AFB2
, AFG1 and AFG2) are 4 [micro]g/kg (EC 2006).
For analysis of total aflatoxins (AFB1, AFB2
, AFG1 and AFG2) and ochratoxin A (OTA) standard methods using Immunoaffinity chromatographic sample extract cleanup and HPLC with fluorescence detection, following international official standards were used as described by Sultana et al.
The mycotoxins analyzed were AFB1 AFB2
AFG1 and AFG2 in 484 healthy adults.
VICAM, a world leader in food safety diagnostics, has announced that AOAC International has awarded Official Method First Action status to the protocol: "Determination of Aflatoxins B1, B2, G1 and G2 and Ochratoxin A in Ginseng and Ginger by Multitoxin Immunoaffinity Column Clean-up and Liquid Chromatographic Quantitation." The method, AOAC number 2008.02, is the first ever to be approved for the simultaneous detection of aflatoxins (AFB1, AFB2
, AFG1 and AFG2) and ochratoxin A.
Aspergillus flavus is the predominant producer of aflatoxins B1 (AFB1) and B2 (AFB2
Mycotoxins include the most widely studied aflatoxins B1 (AFB1), B2 (AFB2
), G1 (AFG1) and G2 (AFG2).
In the cultures of Aspergillus flavus Link isolates, AFB1, AFB2
and AFG1 were detected in 78%, 71%, and 36% of the isolates.
For maize and sorghum, total aflatoxins (AFB1, AFB2
, AFG1 and AFG2) were measured in parts per billion (ppb) using total aflatoxin assay, Cat.
As for the mycotoxin analysis, out of 40 isolates of Aspergillus, 29 isolates produced AFB1 and only two isolates produced AFB2
. Fifteen out of 16 Fusarium isolates produced MON, 12 isolates produced BEA and all isolates produced FB1, but none of them produced ZEA.
Standard calibration solutions showed excellent repeatability and reproducibility for AFGl, AFBl, AFG2 and AFB2
respectively as shown in Fig.