AFB2Aflatoxins B1 and B2
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For analysis of total aflatoxins (AFB1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA) standard methods using Immunoaffinity chromatographic sample extract cleanup and HPLC with fluorescence detection, following international official standards were used as described by Sultana et al.
AFB2 was detected in only two samples, each one of fresh and ensiled maize fodder (1.
HAZ, WAZ and WHZ were used as the dependent variables, and AFM and total aflatoxin (AFB1, AFB2, AFG1 and AFG2) consumption/kg, protein/kg, fat/kg, carbohydrates/kg, gender, whether the child is currently breast fed, and location (Dagoretti or Korogocho) were added as independent variables.
The sample peaks were compared with the standard curve to determine the concentration of AFB1 and AFB2 produced (Khayoon et al.
flavus did not produce AFB1, and 35 isolates did not produce AFB2.
The analysis of AFB1 and AFB2 using UFLC analysis, eight isolates (P14C, C98C, M108C, M109C, B119C, B124C, T145C and Q198C) were negative for the production of AFB1 and AFB2.
flavus to produce AFB1 and AFB2 supports the finding that A.
In some nut samples the levels of AFG1 and AFB2 fall under LOD, but the levels up to which these toxins were detected, the degradation followed first order kinetics.
There was a proportional decrease in AFB2 with increase in UVC exposure time.
Our results are partially inconsistent with Basaran, 2009 who reported that a UV light of 254 nm did not affect AFG2 and AFB2 but significantly reduced AFG1 and AFB1.
For pre column derivatisation TFA (100 uL) was added to the residues or aflatoxin standards to derivatize AFB1 and AFG1 however, AFB2 and AFG2 samples were not derivatised as this is un-necessary.