Patients with sepsis (day 1:45304 [+ or -] 11096 AFUs versus day 4: 45081 [+ or -] 11047 versus day 7: 44871 [+ or -] 19480; ANOVA, p = 0.99) and severe sepsis (day 1: 37181 [+ or -] 2204 AFUs versus day 4: 37513 [+ or -] 1954 AFUs versus day 7: 40116 [+ or -] 2622 AFUs, ANOVA, p = 0.68) did not show any significant change in NETosis over time.
Survivors of sepsis/severe sepsis had greater PMA-induced NETosis on admission than nonsurvivors had (47153 [+ or -] 1559 AFUs versus 34241 [+ or -] 4666 AFUs; Student's t-test p = 0.002; see Figure 3).
The receiver operator curve (ROC) of survivors and nonsurvivors demonstrated an area under the curve of 0.70 (95% CI: 0.56-0.86; p = 0.011) with admission PMA-induced NET value of less than 39000 AFUs, having a 56% sensitivity and a 71% specificity for predicting 30-day mortality, whilst for 90day mortality there was a 47% sensitivity and 70% specificity.
Suppression of ex vivo NETosis below 39000 AFUs was performed comparatively with other traditional biomarkers in predicting mortality from sepsis such as the severity of acidosis (SBE) and lactate [30].
Approved Finishing Units (AFUs) must be approved by APHA and must follow strict conditions to reduce the potential risk of disease spread.
AFUs provide a route for cattle keepers to fatten or finish animals from both TB-restricted and unrestricted farms.
president Minette Batters, NFU deputy president, said: "This is an excellent result for farmers with grazing AFUs. The change to the guidance was introduced last August without any consultation with industry, or any apparent disease control justi-fication.