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We screened cDNA for AHFV by real-time PCR with primers targeting the 5' untranslated region of AHFV (12).
IgG and IgM testing for AHFV, CCHV, and RVFV was done with Centers for Disease Control and Prevention (Atlanta, GA, USA) reagents and protocols by using cell culture-derived antigens (7,10,11).
Phylogenetic analysis of the 7 tick isolates and the available homologous sequences of AHFV are presented in the Figure.
To date, AHFV has been isolated only from human samples.
Alkhurma hemorrhagic fever virus (genus Flavivirus, AHFV) was recently identified as the agent of a viral hemorrhagic fever in Saudi Arabia and characterized serologically and genetically as a variant genotype of Kyasanur Forest disease virus (KFDV).
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