Heat stress and AHSP Animals: Mice were purchased from the Laboratory Animal Center, Sun Yet-Sen University of Medical Sciences.
Drugs and agents: AHSP, developed by the Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, was confected with honeysuckle, astragalus, ophiopogon, schisandra, diffusa, bupleurum, and g-aminobutyric acid (GABA) according to a certain proportion (Chen et al.
Animal groups and sample collecting: After 3 days adaptive feeding, mice were randomly divided into four groups: the control group, the AHSP group, the HS group, and the (AHSP+HS) group.
For the control group and the AHSP group, blood was collected without prior heat stress stimulation.
AHSP acute toxicity test: The AHSP acute toxicity test was performed as described previously (Xu, 2001).
In contrast, AHSP administration reduced the activities of LDH and CK and the concentration of LD in the plasma of heat stress treated mice, which, however, were still higher than in the control group and the AHSP group.
Microscopic changes in the structure of mice livers and intestines: As shown in Fig.1, Under a microscope, we found that compared to the control group and the AHSP group, heat stress caused a series of changes in the structure of the livers and intestines.
These observations indicated that AHSP can take protective effects on the livers and intestines of heat stress treated mice.
AHSP acute toxicity test: After drug administration, the AHSP group exhibited normal feeding, behavior, respiration, secretion, and response.
Table.1 Effect of AHSP on the activity of LDH, CK and the content of LD in the plasma of mice under heat stress [?] x+-SD n=6
Table.3 The weight change of mice in each group in AHSP acute toxicity test g[?] x+-SD n=14
 Nonstandard abbreviations: Hb, hemoglobin; AHSP
, [alpha]-Hb stabilizing protein; RPLC, Reversed-phase liquid chromatography; LC, liquid chromatography; ESI MS, electrospray ionization mass spectrometry; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight; [[alpha].sup.Groene Hart], [alpha] chain encoded by the HBA1 allele carrying the C[right arrow]T mutation in codon 119; TFA, trifluoroacetic acid.