(Cell Signalling Technology (CST), 9272S, Leiden, Netherlands), Phospho-Akt (Ser473) antibody (CST 4060S, Leiden, Netherlands), [beta]-Actin Antibody (CST 4967, Leiden, Netherlands) antibodies were used for western blotting.
With the increase of LY294002 concentration, phosphorylation levels of PI3K and AKT
were notably reduced compared with the untreated cells (P<0.05, P<0.01 or P< 0.001, Figure 4A).
It's main players are protein kinase B (Akt
) and the mechanistic target of rapamycin (mTOR).
However, no variations were observed in the total Akt
Study author Charles Hoeffer said, "AKT
is a central protein that has been implicated in a bevy of neurological diseases yet we know amazingly little about it".
We found that cotreated 8-Br-cGMP with hypoxia showed a significant decrease in protein levels of PI3K p110[alpha], PI3K p110[beta], and phosphorylated AKT
in comparison with the hypoxia group.
The mathematical model is constructed by extending the AKT
phosphorylation model in .
Measurement of Akt
, p-Akt, GSK-3[beta], and p-GSK-3[beta] by Western Blot.
In fact, mTORC2 phosphorylates protein kinase C (PKC)[delta], PKC[zeta], PCK[gamma], and PkC[epsilon] that are involved in cytoskeleton assembly and cell migration, besides AKT
on serine 473 [18-21] (Figure 2(c)).
On the other hand, considering the known close relationship between AKT
and SREBP2, the ARRB-dependent signaling pathway may be included in cholesterol regulation.
For dual staining of Akt
and NF-[kappa]B, the sections were stained with a rabbit polyclonal antibody against rat Akt
(1 : 300, Abcam, USA), a rabbit monoclonal antibody against rat NF-[kappa]B p50 (1: 100, Abcam, USA), an Alexa Fluor 594 goat anti-rabbit IgG (1: 200 dilution, Abcam, USA), and an Alexa Fluor 488 goat anti-rabbit (1:200 dilution, Abcam, USA).
In patients with type 2 diabetes undergoing a resistance exercise program, there are increases in PI3K activity, Akt
content, GLUT4, and GS activity.