As shown in Figure 3(a), AlAPS, AlAPS-1, AlAPS-2 and AlAPS-3 expressed the markedly increased scavenging activities on hydroxyl radical dose-dependently.
Apparently, both AlAPS and its purified fractions had obvious scavenging effects against DPPH radicals in concentration-dependent manners.
When the polysaccharides concentrations raised from 0 to 1.0 mg/mL, the reducing power of AlAPS, AlAPS-1, AlAPS2, and AlAPS-3 was 0.459 [+ or -] 0.05, 0.231 [+ or -] 0.11, 0.753 [+ or -] 0.08, and 0.098 [+ or -] 0.13, respectively, indicating that the AlAPS2 had superior antioxidant activities than AlAPS, AlAPS 1, and AlAPS-3 in vitro.
For the [Fe.sup.2+]-chelating activity, as exhibited in Figure 3(d), both AlAPS and its purified fractions showed dose-dependent manners and presented a smooth growth within the concentrations of 0-1.0 mg/mL.
Interestingly, AlAPS and its fractions significantly suppressed the contents of MDA and LPO dose-dependently.
Furthermore, the AlAPS-2 (38.69 [+ or -] 2.05 g/L) showed superior effects when compared to AlAPS (31.72 [+ or -] 1.63 g/L), AlAPS-1 (34.31 [+ or -] 1.34 g/L), and AlAPS-3 (30.78 [+ or -] 0.96 g/L) at a dose of 400 mg/kg, respectively.
The present study exhibited that AlAPS were considered as representative heteropolysaccharides due to the elution purified by DEAE-52 cellulose column and gas chromatograph.