Potential RNA splicing signals in AMDV were present on the GFADV genome (online Technical Appendix Figure 1, www.
The phosholipase 2 motif in the N terminal VP1 region, generally conserved in parvoviruses, was not found in either GFADV not AMDV (online Appendix Figure 3), which suggests that this parvovirus genus uses a different mechanism to escape the endosome during infection (15).
Comparison of NS1 regions showed GFADV shared [approximately equal to] 74% nt and 67% aa similarities with AMDV strains, whereas different strains of AMDV shared >87% nt and 82% aa similarities.
We examined AMDV antibody from each of the 2 patients by countercurrent and line electrophoresis (13).
Antibodies to AMDV were found in his serum at the end of these 10 years and at 2 subsequent measurements after 1 additional year.
Subsequent investigations repeatedly demonstrated anti-AMDV antibodies and AMDV DNA in peripheral blood and bone marrow.
The clinical history, histopathologic features, and molecular findings for the 2 mink farmers exposed to AMDV were similar to those described for AD in mink.
For patient 1, anti-AMDV antibodies persisted 4 years from his last exposure to mink, exceeding the longest reported duration of positive AMDV response in a human in the absence of virus exposure (11).