Similar to that of AMDV, the GFADV genome contained 2 major ORFs.
Sequence analyses confirmed that GFADV was a divergent amdovirus with 76% nt identity with the genome of AMDV. Conserved protein domains typical of parvoviruses were identified in GFADV.
Comparison of NS1 regions showed GFADV shared [approximately equal to] 74% nt and 67% aa similarities with AMDV strains, whereas different strains of AMDV shared >87% nt and 82% aa similarities.
Reports of a possible relationship between AMDV and human infection are rare (9).
We examined AMDV antibody from each of the 2 patients by countercurrent and line electrophoresis (13).
Antibodies to AMDV were found in his serum at the end of these 10 years and at 2 subsequent measurements after 1 additional year.
Subsequent investigations repeatedly demonstrated anti-AMDV antibodies and AMDV DNA in peripheral blood and bone marrow.
The clinical history, histopathologic features, and molecular findings for the 2 mink farmers exposed to AMDV were similar to those described for AD in mink.
For patient 1, anti-AMDV antibodies persisted 4 years from his last exposure to mink, exceeding the longest reported duration of positive AMDV response in a human in the absence of virus exposure (11).