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Expression vectors pHT43-BMP2-M and pHT43-BMP2-D contained the Pgrac strong promoter and signal peptide sequence of amyQ ([alpha] amylase) gene of Bacillus amyloliquefaciens which has been reported for the efficient secretion of recombinant proteins through Sec pathway [26].
The difference resides among the signal peptide, vector, and medium used; pLip signal peptide under the control of HpaII promoter was used in BMP7 production in LBL (Luria Broth + Lactose) medium [15] whereas we have used amyQ signal sequence under Pgrac promoter control with 2x YT medium.
Abbreviations ALP: Alkaline phosphatase amyQ: [alpha]-Amylase B.
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