AP-PCR

AcronymDefinition
AP-PCRArbitrarily Primed Polymerase Chain Reaction Technique
References in periodicals archive ?
Truong y colaboradores (38) concluyen que, debido a que la AP-PCR es rapida y reproducible, es de gran valor para distinguir especies de S.
mutans, registro #1091; 2) el Centro de Investigaciones Odontologicas, Facultad de Odontologia, Pontificia Universidad Javeriana, titulo Genotipificacion por AP-PCR de Streptococcus mutans aislados de pacientes con y sin caries dental", registro 3648, y 3) el Departamento Administrativo de Ciencia, Tecnologia e Innovacion (Colciencias), Programa Nacional de Ciencia y Tecnologia de la Salud, titulo Identificacion de cepas S.
Genotypic profiles by AP-PCR of Streptococcus mutans in caries-active and caries-free preschoolers.
Los cuatro cebadores tipo AP-PCR evaluados generaron patrones de bandas con tamanos que oscilaron entre 250 y 2000 pb, el numero total de fragmentos amplificados fue de 42 de los cuales nueve fueron polimorficos, 12 se obtuvieron con el Oligo 1, 13 con el Oligo 2, ocho con el Oligo 3 y nueve con el Oligo 4.
En la figura 3 se muestra el fragmento inferior del dendrograma obtenido con el metodo UPGMA mediante marcadores AP-PCR mostrando los grupos G2 a G6.
roreri de Antioquia basado en el perfil de bandas generado por los marcadores AP-PCR.
The AP-PCR primers generated a higher degree of polymorphism in sunflower than did RAPD primers (data not shown) and resulted in distinct and clear patterns.
By AP-PCR fingerprinting, we demonstrated in the present work that interspecific hybridizations are in general a very useful tool for the transfer of genomic portions from the wild species into the cultivated sunflower.
After interspecific hybridization, techniques such as AP-PCR markers can be used for monitoring the introgression of wild genome portions into sunflower material during the backcross procedure.
The patterns obtained by AP-PCR and PFGE (Xba I- and Spe I-digests) were almost indistinguishable; however, when DNA of the same strains was analyzed by PFGE after digestion with Avr II, differences were detected.
Since the AP-PCR and PFGE typing results suggested that a single clone was the causative agent in all cases, the question of its possibly increased virulence arose.