AP-PCRArbitrarily Primed Polymerase Chain Reaction Technique
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A distinct pattern was observed in all 18 strains when they were analyzed by AP-PCR (data not shown), which suggests close genetic relatedness.
The patterns obtained by AP-PCR and PFGE (Xba I- and Spe I-digests) were almost indistinguishable; however, when DNA of the same strains was analyzed by PFGE after digestion with Avr II, differences were detected.
Since the AP-PCR and PFGE typing results suggested that a single clone was the causative agent in all cases, the question of its possibly increased virulence arose.