AP-PCR

AcronymDefinition
AP-PCRArbitrarily Primed Polymerase Chain Reaction Technique
References in periodicals archive ?
mutans en ninos con caries dental y que la genotipificacion con AP-PCR es util para demostrar la diversidad de genotipos (25-29).
En la AP-PCR, con el fin de incrementar el poder discriminatorio de la tecnica, se han utilizado diferentes imprimadores (28,29,32,33).
mutans Isolates by AP-PCR. AP-PCR assays were performed with the arbitrary primer OPA 02 (57-TGCCGAGCTG-37) 19].
Images of AP-PCR fingerprints were captured by a digital camera (Canon Inc., Tokyo, Japan) and stored in Image File Format for visual analysis.
Para analise de PCR com oligonucleotideos arbitrarios (AP-PCR), foram utilizados tres oligonucleotideos derivados de marcadores microssatelites: [(GACAC).sub.3], [(GACA).sub.4] e [(CAG).sub.5].
Trinta loci AP-PCR foram identificados nos isolados estudados, independentemente da intensidade, estando no intervalo de 400 a 1600pb.
Las reacciones de amplificacion para los marcadores AP-PCR se realizaron en un volumen final de 20 [micron]l con 1,5 [micron]l de ADN en una dilucion 1:10, 2 [micron]l de dNTP (1,25 mM), 1 [micron]l de cebador (20 pM/ [micron]l), 11,3 [micron]l de agua ultrapura, 2 [micron]l del buffer (10X) de la enzima, 2 [micron]l de Mg[Cl.sub.2] (25 mM) y 0,2 [micron]l de Taq polimerasa (5U/[micron]l) (Afanador et al, 2003; Freeman et al, 1993; Freeman et al, 1995).
After an initial screen of 160 primers, i.e., short RAPD primers (10 bp) and longer AP-PCR primers (17-20 bp), 20 AP-PCR primers were used for the amplification of random DNA sequences (Table 2).
PCR assays were performed by using approximately 10 ng per reaction tube, except for AP-PCR, in which 25 ng were employed.
A distinct pattern was observed in all 18 strains when they were analyzed by AP-PCR (data not shown), which suggests close genetic relatedness.