Erber, "Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP
complexes)," Journal of Histochemistry & Cytochemistry, vol.
Prosecutor II Ricky Jones Macabaya indicted Adrian Adlawan, Irenea Victoria Clifford, Tadeusz Marian Anthony Mikiel, Jamestephen Vasquez, Rowena Apaap
and Neal Ballesteos for the aforesaid violation of Republic Act No.
Using an Autostainer: Universal Staining System Version 2.0 (DAKO, Carpinteria, CA), cross-sections were processed using the alkaline phosphatase anti-alkaline phosphatase (APAAP
method)20 against the antigens: (1) CD68 located in the lysosomes of macrophages; (2) NP57, the neutrophil granule protein elastase (DAKO, Carpinteria, CA); (3) CD20, an intracytoplasmic epitope on the majority of B-lymphocytes (DAKO); and (4) CD8, glycoproteins on the surface of cytotoxic T-lymphocytes (Vector Labs, Burlington, Ontario).
During a second and third incubation step, cells were incubated for 30 min with 30 [micro]L of an unlabeled rabbit anti-mouse antibody (Dako Corporation) diluted 1:20 in 10 g/L BSA in 1x phosphate-buffered saline and 30 [micro]L of the APAAP
complex (Dako Corporation) diluted 1:20 in 10 g/L BSA in 1 x phosphate-buffered saline, respectively.
For specific immunostaining of macrophages, a mouse monoclonal anti-rat ED1 antibody (Serotec, #MCA341R, Oxford, UK) was used and APAAP
(alkaline phosphatase-antialkaline phosphatase) IHC technique was applied.
Florencio Ortilla identified the suspect as Rejesus Apaap
Jr., resident of No.
Mackay (Brisbane, Australia) for critical reading of the manuscript and to Monika Seiffert for expert analyses of APAAP
method was used to perform immunohistochemical stains on the bone marrow trephine biopsy.
Examples include peroxidase-antiperoxidase (PAP), alkaline phosphatase-antialkaline phosphatase (APAAP
), labeled streptavidin-biotin peroxidase (LSAB), and avidin-biotin complex (ABC) methods.
For each CL, the presence or absence of the antigen assessed was confirmed by established methods, namely Western blot and an immuno-alkaline phosphatase method (APAAP
) (17, 21, 24-27).
Positive prostate cells were visualized by the alkaline phosphatase anti-alkaline phosphatase staining procedure, following manufacturer's recommendations (Dako APAAP