Analysis of DE and plasma was performed with UPLC-MS/MS system equipped with a system controller (SCL-10 ATVP
; Shimadzu), a binary pump (LC-10 ATVP
; Shimadzu), a UV-VIS detector (SPD-10 AVP, Shimadzu), a column oven, and an auto injector (SIL-10A; Shimadzu) with an electrospray ionization (ESI) interface.
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High performance liquid chromatography (HPLC) of the samples was performed with the HPLC system (Shimadzu Corporation, Kyoto, Japan) equipped with two Shimadzu LC-10 ATVP
reciprocating pumps, a variable UV-VIS detector (Shimadzu SPD-10 AVP) and a Winchrom integrator (Winchrom).
Method development and validation were performed with a Shimadzu (VP Series) with UV and PDA detector system controller was SCL-10 A VP, pump model LC-10 ATVP
. Detector model SPD-10A VP and autoinjector auto sampler SCL-10 ADVP.
Para el analisis se utilizo un cromatografo liquido de alta resolucion (Shimadzu[R], Kioto, Japon), que consiste en: un sistema controlador (modelo SCL-10), un modulo liberador de solventes (modelo LC-10 Atvp
) con unidad de gradiente de baja presion (modelo FCV-10 Alvp) y desgasificador (modelo DGU-14 A).