AVEC-DICAllen Video Enhanced Contrast-Differential Interference Contrast Microscopy
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Rhodamine-phalloidin (0.5 [micro]M) was added to stain the actin filaments, and the myosin-II motor activity was monitored by AVEC-DIC and fluorescence microscopy.
Actin bundles detectable by AVEC-DIC microscopy assembled spontaneously in the meiotic phase extracts and formed interactive three-dimensional networks or pseudo-contractile rings by a mechanism of self-organization (6).
Fluorescence microscopy was used to view rhodamine-phalloidin stained actin filaments in the extracts, and AVEC-DIC microscopy was used to assay vesicle transport on actin filaments.