For AZFa, AZFb
and AZFc regions of the Y chromosomes, PCR reactions were performed by using sY14, sY86, sY127 and sY254 regions primers, respectively.
Multiplex A: ZFY: 495 bp, sY84: 326 bp (AZFa), sY134: 301 bp (AZFb
), sY255: 126 bp (AZFc)
The DNA was then subjected to polymerase chain reaction (PCR) and assayed using the following tagged sequences: ZFY (control), sy14 (SRY); AZFa specifically, sy84, sy86, sy625, sy114; AZFb
specifically, sy117, sy127, sy129, sy134, sy143; AZFc specifically, sy152, sy147, sy254, sy255, sy157; and finally heterochromatin sy160.
Among the infertile cases, there were 4 (3.48%) AZFa, 2 (1.74%) AZFb
, 3 (2.61%) AZFc and 7 (6.09%) AZFd region microdeletions.
La delecion del AZFb
fue confirmada usando PCR y pruebas de sourthern blotting (11), confirmando la su presencia en tres generaciones.
deletions predict the absence of spermatozoa with testicular sperm extraction: Preliminary report of a prognostic genetic test.
Protein-encoding gene families on the AZFb
region include RBMY, PRY, and CDY2 which are expressed only in the testis.
In 10 of the cases AZFc deletion, in 1 case AZFb
deletion and in 1 case both deletions were identified.
The increase of primary spermatocytes was particularly shown in AZFb
and AZFc microdeletions (9,10), but with fundamentally different meiotic pictures.
The AZF region is divided into three nonoverlapping subregions called AZFa, AZFb
, and AZFc, all of which are required for normal spermatogenesis.
In the AZF region, three loci termed as AZFa, AZFb
and AZFc have been identified.
High prevalence of AZFb
microdeletion in Iranian patients with idiopathic non-obstructive azoospermia.