AcLDLAcetylated Human Low Density Lipoprotein
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In both groups, the number of clusters dissipated within 7 days of culture, giving rise to spindle-shaped cells (Figure 4A), which stained positive for Sca-1 and Flk-1, as well as for acLDL and UE-lectin (Figure 4C).
Cross-reactivity of anti-CLDL antibody to gLDL, acLDL, and oxLDL, evaluated by indirect ELISA, was extremely low (ratios, 380 for CLDL/gLDL, 810 for CLDL/acLDL, and 2630 for CLDL/ oxLDL).
Several chemically modified forms of LDL have been reported since the 1970s: acLDL (21, 45), gLDL (46, 47), oxLDL (20,41), and cLDL (8,11,27).
Total cellular RNA from unloaded (control) macrophages and from macrophages that have been loaded with cholesterol by using either AcLDL or OxLDL is isolated by means of a standard procedure using guanidinium isothiocyanate [7], and differential display is performed with a commercially available kit (Display Systems, Los Angeles, CA) and a standard protocol using AmpliTaq DNA polymerase (Perkin-Elmer, Norwalk, CT) and [alpha]-[[sup.