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AMAmplitude Modulation
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References in periodicals archive ?
[6] Nonstandard abbreviations: EV, extracellular vesicle; AM, acetoxymethyl; CFSE, carboxyfluorescein succinimidyl ester; di-8-ANEPPS, 4-(2-[6-(dioctylamino)-2-naphthalenyl]ethenyl)-1 (3-sulfopropyl)pyridinium; PKH67, Paul Karl Horan 67; EpCAM, epithelial cell adhesion molecule; CD61, integrin, [beta]3; FITC, fluorescein isothiocyanate; APC, allophycocyanin; SEC, sizeexclusion chromatography; mAb, monoclonal antibody; PE, phycoerythrin; MESF, molecules of equivalent soluble fluorochrome; PPV, positive predictive value.
Cell viability was also assessed after 14 and 21 days using Calcein acetoxymethyl (0.1 [micro]g/ml) and propidium iodide (1 [micro]g/ml) and viewed by fluorescence microscopy.
(2R,3R,5R,6R)-2-(Acetoxymethyl)-6-((4R,5R)-5-(methoxycarbonyl)-2,2-dimethyl-1,3-dioxolane-4-carboxamido)tetrahydro-2H-pyran-3,4,5-triyl Triacetate 5b.
5 x [10.sup.7] fibroblasts washed twice with loading buffer ([10.sup.7] mM NaCl, 5mM KCl, 7mM NaHC[O.sub.3], 3mM Ca[Cl.sub.2],1mM MgS[O.sub.4] x 6[H.sub.2]0,20 mMHepes, 10 mM glucose, and 1% bovine serum albumin (BSA)) were incubated at the concentration of [10.sup.6] fibroblasts/mL with 4 mM 1-(2-(4-carboxyphenyl)-6- amino-benzofuran-5-oxy) -2-(2,-amino-5i-methylphenoxy)ethane-N,N,N,,N,-tetraacetic acid (Fura-2) acetoxymethyl ester for 45 min at 37[degrees] C in a 5% C[O.sub.2] humidified atmosphere.
Application of Alamar blue/5-carboxy fluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout.
Calcium was monitored with the fluoroprobe Fluo4 (3 [micro]mol/L concentration, acetoxymethyl ester form, Molecular Probes, Eugene, Oregon), using a Concord system (Wallac/Perkin-Elmer) incorporating a Spectra-Master (Melville, New York) multiwavelength controller and temperature-controlled stage as described earlier.
Effect of benzo(a)pireno and methyl(acetoxymethyl)nitrosamine on thymidine uptake and induction of aryl hydrocarbon hydroxylase activity in human fetal esophageal cells in culture.
The acetoxymethyl ester of calcein is a lipid-soluble fluorogenic diester that passively crosses the cell membrane in an electrically neutral form, when then it is converted by intracellular esterases, which are active only in entire cells, into the negatively charged, green fluorescent calcein [18].
Hoechst, Ethidium Homodimer, and Calcein Acetoxymethyl (AM) (HEC) Staining to Determine Live and Dead Cells.
Twenty-four hours after dissociation, FDB fibers were incubated with 5 /M Fura-2 acetoxymethyl ester (Fura-2 AM; Invitrogen, Eugene, OR, USA) for 30 min at 37[degrees]C, in a buffer containing the following: 125 mM NaCl, 5 mM KCl, 1 mM MgS[O.sub.4], 1 mM K[H.sub.2]P[O.sub.4], 5.5 mM glucose, 1 mM Ca[Cl.sub.2], 20 mM HEPES, and 1% bovine serum albumin, pH 7.4.
[DELTA]pH was monitored by the fluorescent pH probe 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester (BCECF AM) (Beyotime, China).
After contraction was measured following electrical pacing at 2 Hz for 8 sec [Ca.sup.2+] sparks were recorded from myocytes loaded with fluo-4 acetoxymethyl ester (5 [micro]M; Molecular Probes) with a Zeiss LSM 710 inverted confocal microscope at excitation wavelength of 488 nm.