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References in periodicals archive ?
Microscopic examination (acid-fast staining) of 3 sputum samples did not reveal acid-fast bacteria. Additionally, the tuberculin test on the patient proved negative.
Therefore, a second colonoscopy was necessary in which the presence of acid-fast bacteria confirmed mycobacterial infection.
To confirm the presence of acid-fast bacteria, tissue sections were stained using multiple commercially available acid-fast stains (Fite's Stain Kit, American Master Tech Scientific Laboratory Supplies, Lodi, California; Kinyoun's Stain, Gibson Labs, Lexington, Kentucky; Ziehl-Neelsen Stain, Gibson Labs, Lexington, Kentucky).
It should be noted that lectin protein from marine invertebrates interacted with not only Gram-negative but also Gram- positive bacteria [22], including acid-fast bacteria. The bioactivity of lectin may appear to function as a pattern recognition protein specific for Gram-negative and acid-fast bacteria through its interaction with Lipopolysaccharide (LPS), and also as an opsonin to increase the efficiency of hemocyte phagocytosis.
That reflected the very low sensitivity of acid-fast bacteria stain in this setting, which is similar to that reported by other studies.
Histology revealed dermal and subcutaneous abscesses, No fungal elements were identified using acid-fast bacilli and Gomori's methenamine silver stains, but acid-fast bacteria were identified in the abscess cavities.
They then found acid-fast bacteria -- which turn the same color as tuberculosis bacteria when exposed to a particular stain -- in lesions on the spinal column of a pre-Columbian child.
Diagnosis of infective endocarditis in these patients was established by transthoracic echocardiography findings and blood cultures positive for acid-fast bacteria (Table).
A definitive diagnosis is often made by pursuing a parallel testing strategy, including a CBC (severe leukocytosis), radiographic evaluation with evidence of granulomas or hepatomegaly, fecal cytology demonstrating the presence of acid-fast bacteria, and culture and/or PCR testing of the feces, sputum, or tissues.
Biopsy specimens were negative for acid-fast bacteria and mycobacterial growth (again, different solid and liquid media, extended incubation periods).