We also measured the CES2 activity of the colorectal samples by activity-based protein profiling
(ABPP) using a serine hydrolase activity probe fluorophosphonaterhodamine .
Furthermore, the molecular assays that can be used to elucidate the mechanism of action include affinity chromatography, activity-based protein profiling
, label-free techniques, expression cloning techniques and others (7,18).
Antibody arrays can profile enzyme activities in protein extracts and cell culture supernatants and can provide a convenient platform to evaluate activity-based protein profiling
with high sensitivity and specificity and less sample consumption than initial gel-based strategies for assessing the functional state of enzymes.