AdGFPAdenovirus Containing Green Fluorescent Protein
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Cells with elevated ROS levels were significantly more common after treatment with Ad-Bach1 than after AdGFP or NS injection (Figures 1(b) and 1(c)), and Ad-Bach1 treatment was associated with significantly greater levels of cleaved caspase 3 (Figure 1(d)), which promotes apoptosis, and with lower levels of cyclin D1 (Figure 1(d)), which is required for progression through the G1 phase of the cell cycle.
Bach 1 overexpression appeared to promote apoptotic nuclear condensation (Figure 2(a)) and cell apoptosis (Figure 2(b)), and cleaved caspase 3 levels (Figure 2(c)) and intracellular ROS levels (Figure 3(a)) were significantly higher in populations of Ad-Bach1 HMVECs than in AdGFP HMVECs.
Relative to control cells transduced with AdGFP, overexpression of catalase blunted the increases in IL-8 and COX-2 mRNA induced by treatment with 10 [micro]M 1,2-NQ for 4 hr (Figure 3G,H).