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References in periodicals archive ?
Many methods have been used to detect C282Y, including oligonucleotide ligation assays (1), fragment length polymorphism analyses of PCR products after restriction enzyme digestion (5), allele-specific oligonucleotide hybridization assays (2), mutagenically separated PCR assays (10), primer extension assays (11, 12), and allele refractory mutation systems (13, 14).
Multiplex PCR with allele-specific oligonucleotide hybridization was another combined approach (30).
In this field, the combination of solid supports bearing a set of immobilized oligodeoxynucleotide (ODN) probes with the allele-specific oligonucleotide hybridization reaction is a useful tool.
Single-nucleotide polymorphisms have been detected by various methods, including allele-specific oligonucleotide hybridization (1), allele-specific amplification (2), and restriction fragment length polymorphism (RFLP) analysis (3).
The strategies developed to screen HH chromosomes for the C282Y (845A) mutation include allele-specific oligonucleotide hybridization (4), restriction enzyme analysis (5), and oligonucleotide ligation assays (3).
Sequencing of the PCR products from 20 randomly selected samples showed 100% concordance with the results obtained from PCR and allele-specific oligonucleotide hybridization on microtiter plates (data not shown).
PCR with allele-specific oligonucleotide hybridization (11, 14, 25), amplification with sequence-specific primers (26-29), ASRA (18, 30), and single-strand conformation polymorphism (31) have been used to genotype the PAI1 promoter polymorphism.
Many methods have been reported for the study of BRCA mutations, including allele-specific oligonucleotide hybridization (8, 9), allele-specific PCR (10), PCR-mediated site-directed mutagenesis (11,12), heteroduplex analysis (HDA) (13-15), single-strand conformation polymorphism (14,16), and the protein truncation test (14,15).
PiZ and PiS genotypes may be determined by DNA-based methods such as restriction fragment length polymorphism, allele-specific oligonucleotide hybridization, allele-specific amplification, direct sequencing, dual-color detection by ligase-mediated analysis, temperature or denaturing gradient gel electrophoresis, and PCR-mediated site-directed mutagenesis (11-19).
For allele-specific oligonucleotide hybridization, this fraction is ~3-10%.
They are investigated by various methods, including restriction enzyme digestion, direct sequencing, use of an amplification refractory mutation system, and allele-specific oligonucleotide hybridization (4).
The oligonucleotide probes used for allele-specific oligonucleotide hybridization were essentially those described by Owerbach et al.