(redirected from Allele-Specific Oligonucleotides)
ASOAddress Supporting Organization
ASOApp Store Optimization (mobile application strategy)
ASOAtlanta Symphony Orchestra
ASOAnd So On
ASOAdministrative Services Only
ASOArmed Security Officer
ASOAnkle Stabilizing Orthosis (support brace; various companies)
ASOAmaury Sport Organisation (France)
ASOAir Support Operations (US DoD)
ASOAircraft Shopper Online
ASOArterial Switch Operation (cardiology)
ASOAuthorized Shipping Outlet (UPS)
ASOAdministrative Services Organization
ASOAviation Safety Officer
ASOApplication Service Object
ASOAdvanced Security Option
ASOAssembler Object
ASOAlgemeen Secundair Onderwijs (Dutch)
ASOAlabama Symphony Orchestra
ASOAnti-streptolysin O
ASOAIDS Service Organization
ASOAccount Security Officer (various companies)
ASOAdelaide Symphony Orchestra
ASOAdvanced Security Option (Oracle)
ASOAutomatic Shut Off
ASOAssociation for the Study of Obesity
ASOArteriosclerosis Obliterans
ASOAlbany Symphony Orchestra (Albany, NY, USA)
ASOArkansas Symphony Orchestra
ASOAfter School Online
ASOAllele-Specific Oligonucleotides
ASOAustralian Society of Orthodontists
ASOAviation Supply Office(r)
ASOAviation Supply Office (Navy)
ASOAll State Orchestra (Colorado)
ASOAdministrative Service Outsourcing (various locations)
ASOAustralian Certified Organic
ASOAnvil Shaped Object
ASOAlton Symphony Orchestra (Godfrey, IL)
ASOAnalog Switch-Off (TV)
ASOArbitrary Slice Ordering
ASOAutomated Systems Operations
ASOAnkara Sanayi Odasi
ASOAnalog Shut Off
ASOAustralian Space Office
ASOAsheville Symphony Orchestra (North Carolina)
ASOAnderson Symphony Orchestra (Anderson, Indiana)
ASOAstronomical Search for Origins
ASOAcademic Services Office
ASOAugust Symphony Orchestra (Bloomfield, NJ)
ASOAurora Symphony Orchestra (Aurora, CO)
ASOAdvanced Solar Observatory
ASOAir Surveillance Officer
ASOAcoustic Sensor Operator (ASW Helicopters)
ASOAstronomía Sigma Octante
ASOAssistant Scientific Officer
ASOAutomatic Stop Order (medication)
ASOArteriosclerotic Obliterans
ASOAdditional Settlement Obligation
ASOAcadémie des Sciences Occultes (French: Academy of Occult Sciences)
ASOAntisense Oligomer
ASOAnkle Stabilizing Orthotic (support brace)
ASOAssistant Supply Officer (Royal Australian Navy)
ASOArmy Safety Office
ASOAs Subrogee Of (legal designation)
ASOAmerican Society of Osseointegration
ASOArea Security Operations
ASOAcquisition Support Organization
ASOAssociation Sportive d'Ollainville (French: Ollainville Sports Association; Ollainville, France)
ASOApparent Successful Offeror
ASOArea Supply Officer
ASOAtomic Spin Orbital
ASOAutomatic Safety Override (locomotive control system)
ASOAmerican Standards Organization
ASOAcademic Sector Operations
ASOAssistant Secretary's Office
ASOAmerican Society for Oceanography
ASOApproximate Simultaneous Orthogonal
ASOAmmunition Supply Officer
ASOAdministration Support Office
ASOAutomotive Safety Offices (Ford)
ASOAssault Supply Officer
ASOAdditional Services Offering
ASOAutomatic Send Only
ASOAnnual Service Order
ASOAmerica Support Organization
ASOActing Station Officer
ASOAuthorized Signing Officer
ASOAlternate Service Office
ASOAfghanistan Solidarity Organisation
ASOAmbler Symphony Orchestra (Ambler, PA)
References in periodicals archive ?
The classic methods (2, 3) include restriction fragment length poly morphism analysis (4), dot-blot and reverse dot-blot with allele-specific oligonucleotide probes (5, 6), amplification refractory mutation system (7), multiplex amplification refractory mutation system (8), competitive oligo priming (9), denaturing gradient gel electrophoresis (10), and direct DNA sequencing.
The ability of the allele-specific oligonucleotide to differentiate between the wild-type and the mutant alleles is determined by the hybridization conditions, the nucleotide sequence that flanks the single-nucleotide polymorphism (SNP), and the secondary structure of the target sequence (23).
Several high-throughput methods are currently in use for typing SNPs, including high-density chip arrays for allele-specific hybridization analysis (7, 8), the homogeneous 5' nuclease (TagMan) assay (9,10), the dye-labeled oligonucleotide ligation (DOL) assay (11), primer extension assay with detection by fluorescence polarization (FP) (12) or mass spectrometry (13), the Invader[R] assay (14), and the homogeneous molecular beacon allele-specific oligonucleotide (ASO) assay (15).
Multiplexed genotyping of [beta]-globin variants from PCR-amplified newborn blood spot DNA by hybridization with allele-specific oligonucleotides coupled to an array of fluorescent microspheres.
Several methods have been developed for apoE typing at the DNA level, using genomic DNA for Southern blots with allele-specific oligonucleotide probes (14, 15).
Apolipoprotein E genotyping using the polymerase chain reaction and allele-specific oligonucleotide probes.
In reaction A, the allele-specific oligonucleotide perfectly complemented the wild-type allele, whereas in reaction B the allele-specific oligonucleotide complemented the [INCL.
For the dot blot, 2 [micro]L of the second PCR reaction was denatured, blotted onto a nylon membrane, and hybridized with 5'-end labeled allele-specific oligonucleotides complementary to the sequence containing five thymidines (5'-TGTGTGTGTTTTTAACAG-3'), seven thymidines (5'TGTGTGTTTTTTTAACAG-3'), or nine thymidines (5'-GTGTGTTTTTTTTTTAACAG-3'), respectively (13) (Fig.
A protocol was established by using samples from 75 Italian families with 21-hydroxylase deficiency already genotyped for gross rearrangements by restriction mapping analysis and for point mutations by PCR and either hybridization with allele-specific oligonucleotides (ASOs) or direct sequencing [8,9].
HLA-DQA1 allele-specific oligonucleotides corresponding to the noncoding strand [denoted by (-)] were covalently coupled to unique sets of carboxylate-modified polystyrene microspheres by using water-soluble carbodiimide (EDC).
A method for the detection of the G985 mutation in MCAD deficiency with Eu-labeled allele-specific oligonucleotides has also recently been described [16].
Two systems in this study, the CF Gold and Inno-LIPA assays, used reverse slot-blot hybridization of biotin-labeled PCR products to a membrane strips) with bound allele-specific oligonucleotide probe pairs, complementary to mutant and wild-type CFTR sequences (15).