Similarly, significant reduction (up to 59%) in ascorbate peroxidase
(cAPX) were also noted by the rhizobacterial inoculation both the presence and absence of L-TRP (Fig.
and hydrogen peroxide scavenging enzyme in plants.
Molecular characterization and physiological role of a glyoxysome-bound ascorbate peroxidase
(APX) (EC 126.96.36.199) activity was measured according to the method of Zhu et al.
For analysis of ascorbate peroxidase
(APX) and peroxidase (POD) activities, total soluble proteins were extracted from the leaves of plants with extraction buffer (for APX: 50 mM HEPES, pH 7.0, 0.1 mM EDTA; for POD: 100 mM potassium phosphate, pH 6.0), and protein concentrations were determined by the Bio-Rad protein assay kit.
(APX, EC 188.8.131.52) activity was determined according to the method of Nakano and Asada .
Inactivation of ascorbate peroxidase
by thiols require hydrogen peroxide.
In the ascorbate- glutathione cycle, two key enzymes, ascorbate peroxidase
(APX) and guaiacol peroxidase (GP), are assumed to deal with the detoxification of reactive oxygen species in plants.
Expression of spinach Ascorbate Peroxidase
isoenzimes in response to oxidative stress.
(2005), catalase (Cakmark et al., 1993) and ascorbate peroxidase
(Krivosheeva et al., 1996) activities.
Plant defense systems to reduce the free oxygen in the cellular tissue and repair damaged tissue often can be in the role of antioxidant enzymes superoxide dismutase, catalase, ascorbate peroxidase
, glutathione reductase, glutathione peroxidase, dihydro ascorbat reductase .
Separate assays specific for ascorbate peroxidase
and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase