B-TABroadband Terminal Adapter
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BAPTA-AM (B-TA) was from Molecular Probes (Eugene, OR), and PP2, Ag 1468 (AG 14), and Ly294002 (Ly) were from Calbiochem (San Diego, CA).
During the preactivation phase (3 min), basal levels of phosphorylation were further lowered by ICI, Nys, and B-TA. During the 6 min onward coumestrol activation phase, ICI was never effective at lowering ERK phosphorylation levels.