In this video, the Exo Bacto
Scrub has been pitted against an ordinary scrubber.
Solidified media with bacto
Agar achieved a higher germination percentage with lesser genetic variation.
After 48 h, shoot tips were transferred to a recovery medium (MS supplemented with 2 mg/L BAP and 7 g/L Bacto
The isolates were inoculated into the conical flask containing Bacto
Bushnell Hass broth in artificial sea water along with 2% crude oil and dextrose as additional carbon source .
Two types of agar, marine agar (Difco Marine Broth 2,216 and Bacto
Agar) and nutrient agar (Peptone, 5 g; Yeast extract, 3 g; NaCl, 5 g; Agar, 15 g; Milli-Q water, 1,000 mL) were used for the recovery of aerobic bacteria.
These bacteria were grown in 8 L of liquid media composed of polypepton and Bacto
yeast extract at 5 and 1 g x [L.sup.-1], respectively, for 3 days at 25[grados]C.
Briefly, 20 mL yeast extract, 0.003 g methylene blue and 1 g Bacto
agar were added to 80 mL Lorena grape must.
Plantlets of approximately 2 cm and with one axillary bud were placed individually in polypropylene-capped test tubes (150 x 25 mm) containing 15 mL of 1/2 MS salt solution without plant growth regulator and solidified with 8 g [L.sup.-1] Bacto
The sediment of specimens was inoculated on Sabouraud dextrose agar, Sabouraud dextrose agar (Merck, Germany) with cycloheximide (Sigma-Aldrich, USA), paraffin agar (0.05 g FeS[O.sub.4], 0.05 g MgS[O.sub.4]x7[H.sub.2]O, 1 g [K.sub.2]HP[O.sub.4], 5 g N[H.sub.4]Cl, 0.05 g ZnS[O.sub.4], 1 g N[H.sub.4]N[O.sub.3], 0.05 g MnS[O.sub.4], 3 g K[H.sub.2]P[O.sub.4], 17 g Bacto
Agar and 1 liter distilled water) and McClung's carbon-free broth tube (0.5 g MgS[O.sub.4]x7H, 2 mg ZnS[O.sub.4], 10 mg Fe[Cl.sub.3], 8 mg Mn[Cl.sub.2] x 4[H.sub.2]O, 0.8 g [K.sub.2]HP[O.sub.4], 2 g NaN[O.sub.3], 1 liter distilled water, pH 7.2) with placement of a paraffin coated glass rod.
They were cultured in 10 mL of tryptic soy broth (TSB; Bacto
, Becton Dickinson, MD, USA), and incubated at 35[degrees]C for 2 h.
corrodens fueron sembradas en agar Brucella (BBL Microbiology Systems, Cockeysville, Md.) enriquecido con 0,3% p/v de Bacto
agar, 0,2% p/v de extracto de levadura, 5% v/v de sangre de cordero desfibrinada, 0,2% v/v de sangre hemolizada, 0,0005% p/v de hemina y 0,00005% p/v de menadiona e incubada en atmosfera de anaerobiosis con 9-13% de C[O.sub.2] a concentraciones por debajo del 1% de oxigeno (Anaerogen, Oxoid, Hampshire, England) a 36[grados]C durante 7 dias.
harveyi, which is an important pathogen of aquacultured Pacific white shrimp, was cultured at 30 [degrees]C in stirred suspension culture in sea-water complete culture medium (seawater, 750 ml; distilled water, 250 ml; Bacto
tryptone, 5 g; yeast extract, 1 g; glycerol, 3 ml) and Difco Marine Broth 2216 (BD Biosci-ence) and was used when in log-phase growth.