Fractions were assayed for arginine ester hydrolase activity with the synthetic substrate BAEE ([alpha]-N-benzoyl-L-arginine ethyl ester).
Kallikrein is known to possess arginine ester hydrolase activity and thus is able to cleave the ethyl ester group from the synthetic substrate BAEE ([alpha]-N-benzoyl-L-arginine ethyl ester).
Fractions containing 59% of the total BAEE hydrolase activity from the second G-75 column were pooled and rechromatographed on a third G-75 column (50 mM Tris-HCl, 50 mM KCl, pH 7.
Fractions containing 92% of the total BAEE hydrolase activity were applied to a DEAE Sephadex A-50 ion exchange column (2.
In designing the new turret, Vickers made use of a simulator, which it brought to BAEE.
By the standard of BAEE exhibitions during the 1980s, the number of new products was smaller than normal.
Several technology demonstration units operating in different frequency bands have now been built and tested, and the unit on display was delivered to the Royal Signals and Radar Establishment (RSRE) several years ago, but had been borrowed back to make its public debut at BAEE.
My predecessor had been very active in BAEE
and I continued with his contacts.
The inhibitory activities on bovine pancreatic trypsin and bovine-chymotrypsin were determined by measuring the remaining hydrolytic activity toward specific substrates, BAEE
and BTEE, respectively, after pre-incubation with PDTI for 10 min.
I first met Philip about 20 years ago at the Martineau Environmental Studies Centre in Birmingham, where BAEE
(the Birmingham branch of NAEE) used to hold its meetings after school for local teacher members.