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BALFBroncho Alveolar Lavage Fluid
BALFBoston Adult Literacy Fund (Boston, MA)
BALFBay Area Lupus Foundation (San Francisco, CA)
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The BALF was recovered manually by gentle aspiration with a disposable syringe after each infusion; the recovery rate of BALF was more than 80%.
The concentrations of IL-17 and IL-35 in BALF of OVA-induced asthmatic mice were measured by commercial ELISA kits according to the manufacturer's instructions (Cusabio Biotech, China).
After the blood had been sampled, the right lung was ligated at the bronchi with surgical wire and BALF was collected by washing the left lung with 3 separate aliquots of 2 ml saline at 37[degrees]C through a tracheal cannula.
In terms of total inflammatory cells, IAV inoculation not only caused a significant increase in the total viable leukocytes in BALF from day 2 but also significantly increased the percentage of neutrophils in BALF in all infected groups (Figures 2(b) and 2(c)).
Before dissection, the bronchoalveolar lavage fluid (BALF) was performed by twice instillation of 0.8 mL ice-cold PBS then centrifuged (1500 rpm, 5 min, 4[degrees]C).
IL-4, IL-6, IL-10, TNF-[alpha], and cytokine-induced neutrophil chemoattractant 1 (CINC1) were determined in the BALF, the PeLF, and lungs, livers, and spleen homogenates by enzyme-linked immunoassay (ELISA) using commercial kits (R&D Systems Inc., Minneapolis, MN, USA).
The expression of mRNA for IL-22 BP in subjects with atopic asthma was discovered in mononuclear cells of the BALF after segmental allergen inhalation challenge.
Cytokines and chemokines in serum, BALF, and CF including granulocyte-colony stimulating factor (G-CSF), interferon-gamma (IFN-[gamma]), interleukin- (IL-) 1[beta], IL-6, IL-10, IL-12, IL-13, IL-15, monocyte chemotactic protein- (MCP-) 1, macrophage inflammatory protein- (MIP-) 1a, interferongamma-inducible protein- (IP-) 10, and tumor necrosis factor- (TNF-) [alpha], were measured using a mouse cytokine/ chemokine-magnetic bead panel (Millipore Corp., Billerica, MA, USA) in a Luminex 100 system (Millipore Corp.).
Besides, a vortex was done for the remaining BALF samples and the supernatant was used for GM detection by using the Platelia Aspergillus EIA (Bio-Rad).