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BAPTABearing and Power Transfer Assembly
BAPTABay Area Psychological Testing Associates (San Francisco, CA)
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After incubation of MDCK-C11 cells for 10 min with the gentamicin solution containing 50 mM BAPTA, an intracellular calcium chelator, the [[[Ca.sup.2+]].sub.i] decreased to near baseline values (96.18 [+ or -] 1.03%).
As animals mature, at P14 calyces, vesicle endocytosis is no longer attenuated by 10 mM EGTA or 1 mM BAPTA (Fig.
There was significant recovery of GFP-TRPC3 after photobleaching in the plasma membrane region which was blocked by brefeldin-A, BAPTA and tetanus toxin.
The isolated hippocampus was homogenized with a glass/Teflon homogenizer in 200 [micro]l of lysis solution (20 mM BAPTA; 10 mM MOPS-Tris, pH 7.5; leupeptin 100 [micro]g/ml; and pepstatin 50 [micro]g/ml).
To induce ER [Ca.sup.2+] store depletion, we added 20 mM 1,2-bis(o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid (BAPTA), a strong [Ca.sup.2+] chelator, and 20 [micro]M I[P.sub.3] to the pipette solution.
This phenomenon occurred with a high concentration of the [Ca.sup.2+] chelator BAPTA in the recording pipette, indicating that group I mGluR activation can trigger eCB-STD in a [Ca.sup.2+]-independent manner.
Moreover, treatments with calcium channel blockers and the calcium chelator BAPTA inhibit GVBD in calcium-containing artificial seawater (ASW) but fail to prevent GVBD in SW-stimulated oocytes (Stricker and Smythe, 2000).
To avoid disruption of cell adhesion with BAPTA, the medium was replaced with culture medium of parallel cultures treated at the same time to maintain the soluble factor released from microglia.
The GluN2B receptor antagonist RO25-6981 and the global NMDA receptor antagonist AP-5 were obtained from Tocris Bioscience (Ellisville, MO, USA), while the membrane-impermeable calcium chelator BAPTA was procured from BioMol (Plymouth, PA, USA).
By contrast, bath application of an inhibitor of PKC (Go6983) or injection of BAPTA into the postsynaptic motor neuron had no significant effects.