000), indicating that overexpression of BASP1 could induce G1 arrest.
The effects of BASP1 overexpression on apoptosis of BHT101 and KMH-2 cells were further examined using Annexin V/PI assay.
The effects of BASP1 overexpression on migration were examined using Transwell assay.
sup], In the present study, we investigated the biological activities of BASP1 in thyroid cancer through in vitro and in vivo functional studies.
BASP1 expression is found to be downregulated in several cancers including hepatocellular carcinoma, prostate cancer, and melanoma.
For one thing, overexpression of BASP1 can inhibit the expression of cyclin D1, promote the expression of p21 and p27, and induce G1 phase arrest.
In addition, it is documented that WT1 could activate the Wnt/[sz]-catenin signaling pathway and promote epithelial-mesenchymal transition (EMT) as well as migration and invasion in cancers, while BASP1 could block these biological activities.
In summary, BASP1 gene was involved in thyroid cancer cell proliferation, cell cycle, apoptosis, and migration.
BASP1 is a transcriptional cosuppressor for the Wilms' tumor suppressor protein WT1.