Inhibition of Myc-induced cell transformation by brain acid-soluble protein 1 (BASP1).
Background: Brain acid soluble protein 1 (BASP1) is identified as a novel potential tumor suppressor in several cancers.
Brain acid soluble protein 1 (BASP1) is a 23,000 protein originally isolated from brain extracts.
Full-length human BASP1 were excised and cloned into the pcDNA3.1 vectors (Invitrogen, CA, USA).
BASP1 primer sequences were as fellow: forward, 5'-CTT CAG ACT CAA AAC CCG GC-3'; reverse, 5'-ACG GTT TGG TCG GAA TTA GC-3'(684 bp).
To evaluate the effects of BASP1 on proliferation of BHT-101 and KMH-2 cells in vivo , mouse models with BHT-101 and KMH-2 xenografts were used.
To determine the effect of BASP1 on the cell cycle of anaplastic thyroid cancer, BHT-101 and KMH-2 cells were seeded in a 6-well plate overnight, then pcDNA-BASP1 was added and incubated with cells for 48 h.
BASP1 expression was analyzed in thyroid cancer tissues and cell lines by immunohistochemistry, RT-PCR, and Western blotting.
As shown in the results of RT-PCR and Western blotting analysis [Figure 1]f, the expression of BASP1 mRNA and protein levels were absent in BHT101 and KMH-2 cells.
The effects of BASP1 overexpression on proliferation in vitro were examined using WST-1 assay.