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The bacterial suspension was mixed with 3% bovine serum albumin (1:1), and 1 mL of the resulting cell suspension was mixed with 4mL of a solution containing PHMB (Hangzhou LC-Chem Co., Ltd., Hangzhou, China), BCDMH (Taixing Jiansheng Fine Biological Technology Co., Ltd., Taixing, China), or both (i.e., PB) and incubated for 3 min.
Then, 10x PB (1x PB indicates 10 mg/L PHMB + 10 mg/L BCDMH) was sprayed on the paper pads one time.
PB (4x) brought about log reduction (LR) = 5.18, larger than that by 600 mg/L PHMB (LR = 4.31) or that by 640 mg/L BCDMH (LR = 4.32).
Mouse lymphoma L5178Y cells lacking spontaneous mutations were exposed to appropriate concentrations of PB (0.8~6.4 [micro]g/mL PHMB + 0.8~ 6.4 [micro]g/mL BCDMH) based on prescreening.
PB is a combined disinfectant established in our laboratory, and it is formed with PHMB and BCDMH. We have studied the PB bactericidal effects on E.
* DBDMH (1,3-dibromo-5,5-dimethlyhydantoin), sometimes inaccurately referred to as "pure bromine," undergoes a reaction similar to that of BCDMH when added to water.
* BCDMH tablets are not used to a significant extent in outdoor pools because bromine cannot be effectively stabilized against decomposition by sunlight.
That's why most swimming pools rely on 1-bromo-3-chloro-5, 5-dimethyl-hydantoin (BCDMH) tablets that are typically 66 percent bromine and 27 percent chlorine to serve as the oxidizer.
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