Herein, we investigated the in vitro inhibition effects of 2,4,6-trihydroxybenzaldehyde, 3,4-dihydroxy-5-methoxybenzoic acid, Serotonin hydrochloride compounds on GST, AChE, BChE
, [alpha]-glycosidase and [alpha]-amylase enzymes.
The results of AChE and BChE
activity are shown in Table 3.
The effect of BChE
on patient survival was analyzed by the Kaplan-Meier method and Cox proportional hazards model.
Univariate and multivariate linear regression analyses were performed to determine independent correlation factors for BChE
peduncularis Samples DPPH I[C.sub.50] TPC AChE (I%) (c) ([micro]g/mL) (a) (mg GA/g) (b) Essential oil 253.2 32.5 [+ or -] 0.1 45.2 [+ or -] 0.2 BHT 18.5 - - Galantamine - - 95.9 [+ or -] 0.2 Samples BChE
(I%) (c) Essential oil 48.6 [+ or -] 0.2 BHT - Galantamine 88.7 [+ or -] 0.2 (a) I[C.sub.50] value at concentrations of 1000-7.8[micro]g/mL; (b) TPC at a concentration of 1 mg/mL;(c) Percentage inhibition at a concentration of 1 mg/mL; [+ or -] represents SD of three independent experiments (p < 0.05)
The main route of transformation is enzymatic hydrolysis, and plasmatic (BChE
) and hepatic (hCE-1) esterases are the main enzymes responsible for forming its metabolites: ecgonine methyl ester, ecgonine, and benzoylecgonine (figure 1A).
instructions for making the pseudocholinesterase enzyme,[sup] which has a variety of physiological effects such as the metabolism of the cocaine and heroin [sup] and the breakdown of organophosphorus esters.[sup] Recent studies indicated that BCHE
gene was amplified in the cells of lung carcinomas,[sup] which were consistent with our results.
Para efectuar el diganostico diferencial de intoxicacion por OP o CB, seria necesario realizar por lo menos una segunda determinacion de la actividad BChE
en el animal 24 h con poterioridad a la primera, y poder evaluar si se mantiene el mismo porcentaje de actividad (OP) o si ha aumentado (CB) (14).
Kinetic studies of BChE
inhibition was determined by constructing Lineweaver-Burk (LB) plots; reciprocal plots of 1/V versus 1/[S] at different concentrations of substrate S-butyrylthiocholine chloride (1.75-14.00 mM) in the absence and presence of two different concentrations of inhibitors (33.2 and 66.4 [micro]M).
mg/L), whereas, the minimum BChE
activity was 14.90 +- 0.60, 3.10 +- 0.20, 4.48 +- 0.18, 20.41+-0.17,6.91 +- 0.17 and